Infection of SK-N-MC cells, a CD4-negative neuroblastoma cell line, with primary human immunodeficiency virus type 1 isolates.
J Virol
; 70(10): 7290-4, 1996 Oct.
Article
en En
| MEDLINE
| ID: mdl-8794385
ABSTRACT
Most studies looking at CD4-independent infection have used laboratory strains or their respective molecular clones. To determine whether primary human immunodeficiency virus type 1 isolates could infect CD4-negative cells, we obtained a panel of 23 clinical isolates and characterized the early steps of the viral life cycle in SK-N-MC cells, a CD4-negative, galactosylceramide-positive neuroblastoma cell line. Eight of 23 isolates established a nonproductive infection; entry and postentry restrictions were noted in the others. We were unable to correlate the infectivity for SK-N-MC cells with established biological phenotypes, such as syncytium induction, or with genetic classifications, suggesting that pantropism is an independent biological variable.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Antígenos CD4
/
Infecciones por VIH
/
VIH-1
/
Neuroblastoma
Límite:
Humans
Idioma:
En
Revista:
J Virol
Año:
1996
Tipo del documento:
Article
País de afiliación:
Estados Unidos