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In vivo and in vitro trans-cleavage activity of hepatitis C virus serine proteinase expressed by recombinant baculoviruses.
Suzuki, T; Sato, M; Chieda, S; Shoji, I; Harada, T; Yamakawa, Y; Watabe, S; Matsuura, Y; Miyamura, T.
Afiliación
  • Suzuki T; Department of Virology II, National Institute of Health, Tokyo, Japan.
J Gen Virol ; 76 ( Pt 12): 3021-9, 1995 Dec.
Article en En | MEDLINE | ID: mdl-8847507
By the use of recombinant baculoviruses, the trans-cleavage of hepatitis C virus (HCV) non-structural polyprotein was studied. The viral serine proteinase encoded by the NS3 gene was expressed efficiently in insect cells infected with a baculovirus recombined with HCV cDNA corresponding to amino acids 1046-1243 and the signal sequence of the rabies virus G protein. Coinfection studies showed the in vivo trans-cleavage activity of the expressed protein by the use of a recombinant producing NS5 as a substrate. We also found that the partially purified NS3 serine proteinase prepared from the recombinant-infected cells could cleave NS5A/5B substrate. Characterization of the proteinase obtained wil provide basic knowledge on processing of the HCV polyprotein.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Serina Endopeptidasas / Proteínas no Estructurales Virales / Nucleopoliedrovirus / Hepacivirus / Antígenos Virales Límite: Animals / Humans Idioma: En Revista: J Gen Virol Año: 1995 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Serina Endopeptidasas / Proteínas no Estructurales Virales / Nucleopoliedrovirus / Hepacivirus / Antígenos Virales Límite: Animals / Humans Idioma: En Revista: J Gen Virol Año: 1995 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido