Hyaluronic acid synthesis by the malignant fibrous histiocytoma cell line NMSG 10 in vitro and its localization.
Pathobiology
; 64(2): 67-72, 1996.
Article
en En
| MEDLINE
| ID: mdl-8888271
ABSTRACT
Viscous material secreted from NMSG 10 cells cultured from malignant fibrous histiocytoma of human bone was demonstrated using two-dimensional electrophoresis to be glycosaminoglycans (GAGs) consisting of mainly hyaluronic acid (HA), along with small amounts of heparan sulfate (HS) and chondroitin sulfate (ChS). The biosynthesis of HA in a cell-free system was located at the plasma membrane fraction by colloidal-iron-reactive ultrastructural staining. The molecular weight of the HA synthesized by the plasma membrane fraction was estimated to be about 90,000 D by gel filtration. The material secreted by the original tumor tissue of NMSG 10 cells was demonstrated to be GAGs consisting of HA, HS, ChS and dermatan sulfate using two-dimensional electrophoresis. These findings suggest that transformed tumor cells could synthesize HA at the plasma membrane and release it into the stroma of tumor tissue.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neoplasias Óseas
/
Membrana Celular
/
Histiocitoma Fibroso Benigno
/
Ácido Hialurónico
Límite:
Humans
Idioma:
En
Revista:
Pathobiology
Asunto de la revista:
PATOLOGIA
Año:
1996
Tipo del documento:
Article
País de afiliación:
Japón