Prostate-specific human kallikrein (hK2) as a novel marker for prostate cancer.
Prostate Suppl
; 7: 17-24, 1996.
Article
en En
| MEDLINE
| ID: mdl-8950360
ABSTRACT
BACKGROUND:
Although prostate-specific antigen (PSA) has aided significantly in the diagnosis of prostate cancer, more sensitive and accurate assays are needed. Presently, we are developing a sensitive immunoassay for hK2 protein for the detection of prostate cancer.METHODS:
Polyclonal and monoclonal antibodies specific for hK2 were produced and used for Western blot analysis and immunohistochemistry for detection of hK2 protein in serum and human tissues. The reverse-transcriptase polymerase chain reaction (RT-PCR) was utilized to detect hK2 mRNA from patient blood samples.RESULTS:
Western blot analysis demonstrated that the antibodies used are monospecific for hK2. Immunohistochemistry showed that hK2 is expressed only in prostatic epithelia. An RT-PCR study showed that hK2 mRNA would be a useful candidate for early detection of prostatic micrometastasis.CONCLUSIONS:
Monospecific antibodies for hK2 have been developed for detecting hK2 protein. Our studies indicate that hK2 may be a useful marker for prostate cancer.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neoplasias de la Próstata
/
Calicreínas
Tipo de estudio:
Screening_studies
Límite:
Humans
/
Male
Idioma:
En
Revista:
Prostate Suppl
Asunto de la revista:
UROLOGIA
Año:
1996
Tipo del documento:
Article
País de afiliación:
Estados Unidos