An in situ method for determining bacterial survival on food preparation surfaces using a redox dye.
Lett Appl Microbiol
; 23(5): 325-8, 1996 Nov.
Article
en En
| MEDLINE
| ID: mdl-8987715
ABSTRACT
A simple method is described for the direct enumeration of viable bacteria dried on test surfaces. Inoculated surfaces were overlayed with agar and after incubation nitroblue tetrazolium solution (pale yellow) was used to stain colonies (purple) at the agar-test surface interface. Stained colonies could be readily detected and counted even against the opaque background of ceramic tile or stainless steel or when present within opaque films of milk or serum. Recovery of bacterial by this method was approximately fivefold greater than using a conventional swabbing procedure. The method was used to demonstrate the marked effect of the composition of the suspension fluid, in which bacteria were dried, and the length of surface exposure upon bacterial survival.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Bacterias
/
Microbiología de Alimentos
Idioma:
En
Revista:
Lett Appl Microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
1996
Tipo del documento:
Article
País de afiliación:
Reino Unido