[A novel method of concentrating bacterial suspensions for transmission electron microscopy]. / Novyi metod kontsentrirovaniia bakterial'nykh suspenzii dlia transmissionnogo élektronno-mikroskopicheskogo analiza.
Mol Gen Mikrobiol Virusol
; (4): 35-9, 1996.
Article
en Ru
| MEDLINE
| ID: mdl-8999322
ABSTRACT
Presents a new method for making preparations of bacteria and their unculturable forms from suspensions with low concentrations of cells, from 1 x 108 to 1 x 104 per ml. to be examined under transmission electron microscope. The novelty of the method consists in the technique of concentrating bacteria by making a colloid solution consisting of bovine serum albumin and polyethyleneglycol. Associations of protein molecules, polyethyleneglycol, and bacteria, forming in the solution, are sedimented by centrifugation after 48-hour incubation at 4 degrees C. The solidity of the resultant macroscopic sediment is sufficient for studying the cells contained in it under transmission electron microscope.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Salmonella typhimurium
Idioma:
Ru
Revista:
Mol Gen Mikrobiol Virusol
Asunto de la revista:
BIOLOGIA MOLECULAR
/
MICROBIOLOGIA
/
VIROLOGIA
Año:
1996
Tipo del documento:
Article