Ooplasmic round spermatid nuclear injection procedures as an experimental treatment for nonobstructive azoospermia.
J Assist Reprod Genet
; 14(1): 55-62, 1997 Jan.
Article
en En
| MEDLINE
| ID: mdl-9013313
ABSTRACT
PURPOSE:
Our objective was to apply ooplasmic round spermatid nuclear injections for the treatment of nonobstructive azoospermia. MATERIALS Participants were nine azoospermic men who had previously undergone diagnostic testicular biopsy. Spermatogenetic arrest was diagnosed at the round spermatid stage (n = 6) or primary spermatocyte stage (n = 3). A second (therapeutic) testicular biopsy was performed and round spermatid nuclei were recovered from all the participants.RESULTS:
Forty-nine mature oocytes were successfully injected with nuclei and then cultured for 72 hr. Twenty-four embryos were transferred to nine women. No pregnancy was achieved.CONCLUSIONS:
Round spermatids can be recovered from therapeutic testicular biopsy material of men negative for round spermatids in previous routine diagnostic testicular biopsy specimens. Round spermatid nuclear injections may play a role in the treatment of nonobstructive azoospermia.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oligospermia
/
Espermátides
/
Fertilización In Vitro
/
Microinyecciones
Límite:
Adult
/
Female
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Humans
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Male
/
Pregnancy
Idioma:
En
Revista:
J Assist Reprod Genet
Asunto de la revista:
GENETICA
/
MEDICINA REPRODUTIVA
Año:
1997
Tipo del documento:
Article
País de afiliación:
Japón