The application of microwave denaturation in comparative genomic hybridization.
Genet Anal
; 13(5): 129-33, 1996 Nov.
Article
en En
| MEDLINE
| ID: mdl-9021402
ABSTRACT
Comparative genomic hybridization (CGH) is a powerful tool for analyzing unbalanced chromosomal rearrangements in a variety of tissues. However, reproducibility of the technique is poor. We have developed an alternative protocol involving microwave denaturation of the metaphase chromosome preparations prior to the hybridization step. The advantage of this method for CGH is the retention of the morphology of the chromosomes and hence an improved chromosome banding pattern. Furthermore, it results in a consistently strong hybridization which is not dependent on the batch of lymphocytes used to obtain the metaphase chromosome spreads. This procedure has also proved to be applicable to nucleic acid hybridizations in general. The protocol, its application and the results of this method in CGH is discussed. Furthermore preliminary results of this method in paint and DNA probe hybridizations to chromosome spreads and to RNA in tissue sections are presented.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Microondas
/
Desnaturalización de Ácido Nucleico
Límite:
Humans
Idioma:
En
Revista:
Genet Anal
Asunto de la revista:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
/
BIOTECNOLOGIA
Año:
1996
Tipo del documento:
Article
País de afiliación:
Países Bajos