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An optical submicrometer calcium sensor with conductance sensing capability.
Shalom, S; Strinkovski, A; Peleg, G; Druckmann, S; Krauss, A; Lewis, A; Linial, M; Ottolenghi, M.
Afiliación
  • Shalom S; Division of Applied Physics, Hebrew University of Jerusalem, Israel.
Anal Biochem ; 244(2): 256-9, 1997 Jan 15.
Article en En | MEDLINE | ID: mdl-9025941
The identification of chemical species and the measurement of their concentrations with high (submicrometer) spatial resolution are of considerable importance in cell biology. In this article we report the first successful development of a > or = 0.1-micron Ca2+ sensor based on a pulled micropipet, filled with a conducting porous sol-gel glass which was doped with the fluorescent calcium green 1 Ca2+ indicator. Such sensors are potentially capable of measuring Ca2+ concentrations as low as 10(-8) M, in confined volumes, with a three-dimensional resolution which exceeds approximately 0.1 micron. A major advantage of the sensor is its capability to be integrated into a multifunctional probe which will measure chemical analyte concentrations and ion conductance.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Calcio / Técnicas de Sonda Molecular Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Anal Biochem Año: 1997 Tipo del documento: Article País de afiliación: Israel Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Calcio / Técnicas de Sonda Molecular Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Anal Biochem Año: 1997 Tipo del documento: Article País de afiliación: Israel Pais de publicación: Estados Unidos