Target cell lysis by CTL granule exocytosis is independent of ICE/Ced-3 family proteases.
Immunity
; 6(2): 209-15, 1997 Feb.
Article
en En
| MEDLINE
| ID: mdl-9047242
ABSTRACT
Activation of ICE/Ced-3 family proteases (caspases) has been proposed to mediate both the granule exocytosis and Fas-Fas ligand pathways of rapid target cell death by cytotoxic T lymphocytes. In agreement with this model, two peptide fluoromethyl ketone caspase inhibitors and baculovirus p35 blocked apoptotic nuclear damage and target cell lysis by the CTL-mediated Fas-Fas ligand pathway. The peptide caspase inhibitors also blocked drug-induced apoptotic cell death in tumor cells. In contrast, the caspase inhibitors blocked CTL granule exocytosis-induced target apoptotic nuclear damage, but did not inhibit target lysis. These results are consistent with recent demonstrations that granzyme B can activate caspases leading to apoptotic nuclear damage, but show that target cell lysis by CTL granule exocytosis occurs by a caspase-independent pathway.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cisteína Endopeptidasas
/
Linfocitos T Citotóxicos
/
Proteínas del Helminto
/
Caspasas
/
Gránulos Citoplasmáticos
/
Citotoxicidad Inmunológica
/
Exocitosis
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Immunity
Asunto de la revista:
ALERGIA E IMUNOLOGIA
Año:
1997
Tipo del documento:
Article
País de afiliación:
Estados Unidos