Rainbow Universal CPG: a versatile solid support for oligonucleotide synthesis.
Biotechniques
; 22(4): 752-6, 1997 Apr.
Article
en En
| MEDLINE
| ID: mdl-9105628
ABSTRACT
We have developed a universal solid support, termed Rainbow Universal CPG, for use in automated oligonucleotide synthesis. The universal solid support allows any oligodeoxyribonucleotide sequence to be synthesized from a single type of controlled pore glass (CPG) support. Deprotection of oligodeoxyribonucleotides was optimized using 0.5 M LiCl in concentrated ammonium hydroxide. PCR experiments using three different sets of primers proved that the 3'-hydroxyl function of oligodeoxyribonucleotides synthesized from Rainbow Universal CPG was retained. This universal solid support shows promise for replacing the standard nucleoside CPG supports.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oligodesoxirribonucleótidos
Límite:
Humans
Idioma:
En
Revista:
Biotechniques
Año:
1997
Tipo del documento:
Article
País de afiliación:
Estados Unidos