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The in vivo expression patterns of individual type I interferon genes in murine cytomegalovirus infections.
Yeow, W S; Lai, C M; Beilharz, M W.
Afiliación
  • Yeow WS; Department of Microbiology, Queen Elizabeth II Medical Centre, University of Western Australia, Nedlands, Australia.
Antiviral Res ; 34(1): 17-26, 1997 Mar.
Article en En | MEDLINE | ID: mdl-9107382
ABSTRACT
Type I interferon (IFN) family has more than 10 structurally related subtypes of alpha IFN (IFNA) genes and a single beta IFN (IFNB) gene. This study examined the expression of MuIFNA1, A4, A5, A6, A9 and MuIFNB mRNAs in the livers and spleens of MCMV-infected mice at 2, 4 and 6 h post infection. The three strains of inbred mice studied were C57BL/6, B6.C-H-28c and BALB/c. B6.C-H-28c strain is a congenic strain of C57BL/6 genomic background. Detection of the specific mRNAs was by an established semiquantitative procedure based on reverse transcription and PCR amplification followed by differential hybridization to specific oligonucleotides. Similar expression patterns of the type I IFN mRNAs were found in C57BL/6 and B6.C-H-28c strains of mice. However, when the genotype was significantly different (BALB/c), a different expression pattern of IFN mRNAs was seen. Differences in the expression patterns of the type I IFN mRNAs was also seen between the livers and spleens of a given mouse strain. Thus, the present study indicates that mouse genotype appears to be a major determinant of the subtype response pattern seen in vivo and that tissue-type can influence the subtype response pattern seen within a given mouse genotype.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Interferón Tipo I / Muromegalovirus / Infecciones por Citomegalovirus Límite: Animals Idioma: En Revista: Antiviral Res Año: 1997 Tipo del documento: Article País de afiliación: Australia
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Interferón Tipo I / Muromegalovirus / Infecciones por Citomegalovirus Límite: Animals Idioma: En Revista: Antiviral Res Año: 1997 Tipo del documento: Article País de afiliación: Australia