Determination of ofloxacin in human aqueous humour by high-performance liquid chromatography with fluorescence detection.
J Pharm Biomed Anal
; 15(5): 663-6, 1997 Feb.
Article
en En
| MEDLINE
| ID: mdl-9127278
ABSTRACT
A reversed-phase high-performance liquid chromatographic method is described for the determination of ofloxacin in human aqueous humour; the method involves fluorescence detection (excitation at 290 nm; emission at 500 nm) after direct injection of samples. The method utilized a 100 mm x 8 mm i.d. cartridge column packed with 4 microns Novapak C18 with a mobile phase methanol-acetonitrile-0.4 M citric acid (3110, v/v/v) and a flow rate of 1 ml min-1 at ambient temperature. The retention times for the internal standard pipemidic acid and for ofloxacin were 4.82 and 7.32 min respectively. The mean recovery (+/- ISD) from human aqueous humour was 103.24 +/- 4.45% for ofloxacin at 1 microgram ml-1 (n = 6). The within-day and day-to-day RSDs at 0.1 microgram ml-1 and 1 microgram ml-1 were less than 6.71% (n = 6) and the lower limit of reliable determination corresponding to a signal-to-noise ratio of 2.51 was 20 ng ml-1. The assay was shown to be suitable for measuring ofloxacin levels in human aqueous humour samples after topical, oral and intravenous administration.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Humor Acuoso
/
Ofloxacino
/
Antiinfecciosos
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
J Pharm Biomed Anal
Año:
1997
Tipo del documento:
Article
País de afiliación:
Turquía