Characterization of tubular structures composed of nonstructural protein NS1 of African horsesickness virus expressed in insect cells.
J Gen Virol
; 78 ( Pt 5): 1077-82, 1997 May.
Article
en En
| MEDLINE
| ID: mdl-9152425
ABSTRACT
The characteristic tubules that are produced during the orbivirus infection cycle are composed of a major viral nonstructural protein, NS1. To characterize the NS1 gene and gene product of African horsesickness virus (AHSV), a full-length cDNA copy of the NS1 gene of AHSV-6 was cloned and the nucleotide sequence determined. NS1 was highly conserved within the AHSV serogroup with between 95-98% conservation of amino acids among NS1 of AHSV-6, AHSV-4 and AHSV-9. The structure of AHSV NS1 tubules was investigated by in vitro translation of the AHSV-6 NS1 gene followed by expression of the gene in insect cells. The NS1 protein assembled in tubular structures with a diameter of approximately 23 nm and lengths of up to 4 microns. The absence of a ladder-like structure and lower sedimentation value of AHSV NS1 tubules clearly distinguished them from those of bluetongue virus.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas no Estructurales Virales
/
Virus de la Enfermedad Equina Africana
Límite:
Animals
Idioma:
En
Revista:
J Gen Virol
Año:
1997
Tipo del documento:
Article
País de afiliación:
Sudáfrica