Adoptive transfer of gut intraepithelial lymphocytes protects against murine infection with Toxoplasma gondii.
J Immunol
; 158(12): 5883-9, 1997 Jun 15.
Article
en En
| MEDLINE
| ID: mdl-9190941
ABSTRACT
Intraepithelial lymphocytes (IEL) of the gut represent a primary immune barrier against infection by orally acquired pathogens. Naturally acquired infection with Toxoplasma gondii induces the proliferation of CD8+ T cells in both the gut and spleen. Gut-derived CD8alpha/beta+ IEL exhibit MHC-restricted cytotoxicity against parasite-infected enterocytes and macrophages. In a murine model, we demonstrate that the adoptive transfer of IEL obtained from inbred mice at day 11 postinfection is able to protect against a virulent challenge in syngenic recipients. In CBA mice, the parasite cyst load within the brain of the recipients receiving primed IEL was reduced by 90%. In BALB/c and C57BL/6 mice, a 50% decrease in mortality was observed following adoptive transfer of primed IEL. To determine the T cell subset responsible for protective immunity, a purified CD8alpha/beta+ IEL population was isolated from infected mice at day 11 postinfection. These cells were able to protect naive mice by adoptive transfer against a lethal parasite challenge. RNA analysis by reverse-transcriptase PCR revealed that primed CD8alpha/beta+ IEL produce significant message for IFN-gamma, an essential cytokine for host protection against toxoplasmosis. Administration of anti-IFN-gamma at the time of adoptive transfer of primed IEL abrogated protection. The adoptive transfer of these protective IEL was not restricted to the Ld class I locus. These data demonstrate that IFN-gamma-producing IEL may be an important primary barrier against acute and perhaps recurrent infection with T. gondii.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Linfocitos
/
Toxoplasmosis Animal
/
Linfocitos T CD8-positivos
/
Traslado Adoptivo
/
Intestinos
Límite:
Animals
Idioma:
En
Revista:
J Immunol
Año:
1997
Tipo del documento:
Article
País de afiliación:
Francia