Evidence that virion-associated VP1 of avibirnaviruses contains viral RNA sequences.
Arch Virol
; 142(6): 1227-36, 1997.
Article
en En
| MEDLINE
| ID: mdl-9229010
ABSTRACT
The VP1 encoded by genomic segment B of birnaviruses is generally known to exist as a genome-linked protein (VPg) and as a "free" polypeptide of 90 kDa in virus particles. The guanylylation activity associated with infectious bursal disease virus (IBDV) was demonstrated by incubating purified virus in presence of [alpha 32P] GTP; optimum activity in the 90 kDa form of VP1 was seen in low salt concentration in the presence of 4 mM magnesium ions over a wide range of incubation temperatures. The IBDV VP1 was shown to lack guanyl transferase activity. Northwestern (RNA-protein) blot analysis of purified virus using a radiolabelled cDNA probe consisting of 3' and 5' ends of genomic segment B indicated that both forms of virion-associated VP1 contained viral RNA sequences of which those linked to VPg corresponded to the two genome segments and those linked to the 90 kDa VP1 were probably a short oligonucleotide of the terminal viral RNA sequences.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ARN Viral
/
Proteínas del Núcleo Viral
/
Cápside
/
Virus de la Enfermedad Infecciosa de la Bolsa
Tipo de estudio:
Risk_factors_studies
Límite:
Animals
Idioma:
En
Revista:
Arch Virol
Año:
1997
Tipo del documento:
Article
País de afiliación:
Canadá