Molecular dissection of two distinct actions of melatonin on the suprachiasmatic circadian clock.
Neuron
; 19(1): 91-102, 1997 Jul.
Article
en En
| MEDLINE
| ID: mdl-9247266
ABSTRACT
The pineal hormone melatonin elicits two effects on the suprachiasmatic nuclei (SCN) acute neuronal inhibition and phase-shifting. Melatonin evokes its biological effects through G protein-coupled receptors. Since the Mel1a melatonin receptor may transduce the major neurobiological actions of melatonin in mammals, we examined whether it mediates both melatonin effects on SCN function by using mice with targeted disruption of the Mel1a receptor. The Mel1a receptor accounts for all detectable, high affinity melatonin binding in mouse brain. Functionally, this receptor is necessary for the acute inhibitory action of melatonin on the SCN. Melatonin-induced phase shifts, however, are only modestly altered in the receptor-deficient mice; pertussis toxin still blocks melatonin-induced phase shifts in Mel1a receptor-deficient mice. The other melatonin receptor subtype, the Mel1b receptor, is expressed in mouse SCN, implicating it in the phase-shifting response. The results provide a molecular basis for two distinct, mechanistically separable effects of melatonin on SCN physiology.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Núcleo Supraquiasmático
/
Ritmo Circadiano
/
Receptores Citoplasmáticos y Nucleares
/
Receptores de Superficie Celular
/
Melatonina
Límite:
Animals
Idioma:
En
Revista:
Neuron
Asunto de la revista:
NEUROLOGIA
Año:
1997
Tipo del documento:
Article
País de afiliación:
Estados Unidos