Involvement of phosphatidylinositol-3 kinase in prevention of low K(+)-induced apoptosis of cerebellar granule neurons.
Brain Res Dev Brain Res
; 101(1-2): 197-206, 1997 Jul 18.
Article
en En
| MEDLINE
| ID: mdl-9263593
ABSTRACT
Cerebellar granule neurons obtained from 9-day-old rats die in an apoptotic manner when cultured in serum-free medium containing a low concentration of potassium (5 mM). A high concentration of potassium (26 mM) in the culture medium and BDNF can effectively prevent this apoptosis. The survival effects of high potassium and BDNF were additive, and the effect of high potassium was not blocked by addition of anti-BDNF antibody. These observations indicated that these survival effects were independent. To examine which molecules are involved in the survival pathway induced by BDNF or high K+, we used wortmannin, a specific inhibitor of PI-3 kinase. Wortmannin blocked the survival effects of both BDNF and high K+ on cerebellar granule neurons. Furthermore, in vitro PI-3 kinase assay showed that treatment with BDNF or high K+ induced PI-3 kinase activity, which was diminished by addition of wortmannin. These results indicate that different survival-promoting agents, BDNF and high K+, can prevent apoptosis in cerebellar granule neurons via a common enzyme, PI-3 kinase.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Potasio
/
Cerebelo
/
Apoptosis
/
Fosfotransferasas (Aceptor de Grupo Alcohol)
/
Neuronas
Límite:
Animals
Idioma:
En
Revista:
Brain Res Dev Brain Res
Asunto de la revista:
CEREBRO
/
NEUROLOGIA
Año:
1997
Tipo del documento:
Article
País de afiliación:
Japón