Characterization of serotype II polysaccharide antigen of group E streptococci using a monoclonal antibody.
Microbiol Res
; 152(2): 113-9, 1997 Jul.
Article
en En
| MEDLINE
| ID: mdl-9265765
ABSTRACT
A Monoclonal antibody (MAb II-T) specific for serotypes II and V Group E streptococci (GES) was prepared by fusing myeloma cells with spleen cells of mice immunized with whole cells of a serotype II strain. MAb II-T reacted in an enzyme immunoassay (EIA) with whole cells of both serotypes and reacted in gel diffusion test with autoclaved-saline extraction of serotypes II and V. The extract was purified by DEAE-Sephadex A-25, followed by treatment with proteinase K, and further by chromatography with a Sephadex G-200 column. The purified polysaccharide (PS) antigen contained 98.6% carbohydrate and 1.4% protein, but no detectable phosphorus. In hapten inhibition tests using various sugars, D-mannosamine markedly inhibited the precipitin reaction. These results indicated that the antigenic determinant might have a structure similar to D-mannosamine.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Polisacáridos Bacterianos
/
Streptococcus
/
Anticuerpos Antibacterianos
/
Especificidad de Anticuerpos
Idioma:
En
Revista:
Microbiol Res
Asunto de la revista:
MICROBIOLOGIA
/
SAUDE AMBIENTAL
Año:
1997
Tipo del documento:
Article
País de afiliación:
Japón