High pressure modulation of Escherichia coli DNA gyrase activity.
Biochem Biophys Res Commun
; 239(2): 552-6, 1997 Oct 20.
Article
en En
| MEDLINE
| ID: mdl-9344868
ABSTRACT
Elevated pressures greater than 551 bar were found to inhibit the DNA supercoiling activity of Escherichia coli DNA gyrase. A large fraction of the inhibitory effect could be reproduced by preincubation of the enzyme at high pressure prior to enzymatic assay at 1 bar. It is proposed that elevated pressure influences gyrase structure, most likely by promoting the dissociation of its subunits, however, it is also possible that effects on enzyme activity exist.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Presión Atmosférica
/
ADN-Topoisomerasas de Tipo II
/
Escherichia coli
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
1997
Tipo del documento:
Article
País de afiliación:
Estados Unidos