Transcriptional analysis of walleye dermal sarcoma virus (WDSV).
Virology
; 237(1): 107-12, 1997 Oct 13.
Article
en En
| MEDLINE
| ID: mdl-9344912
ABSTRACT
Walleye dermal sarcoma virus (WDSV) is a complex retrovirus associated with dermal sarcomas of walleye that develop and regress on a seasonal basis. WDSV contains, in addition to gag, pol, and env, three open reading frames (ORFs) designated ORF A, ORF B, and ORF C. The polymerase chain reaction technique was used to amplify and clone cDNAs representing subgenomic viral mRNAs isolated from developing (fall) and regressing (spring) tumors. Nine different singly or multiply spliced viral transcripts were identified and all were found to utilize a common 5' leader sequence. This leader sequence is spliced to the pol/env junction or downstream of env to generate singly spliced transcripts. Multiply spliced transcripts contain the 5' leader, the pol/env junction, and sequences derived from the 3' end of the genome. One multiply spliced transcript was isolated with the potential to encode the full-length ORF A protein. In addition, WDSV produced mRNAs that utilize alternative splice acceptor sites which would allow synthesis of five variant forms of the ORF A protein. In contrast, the ORF B protein is postulated to arise from a singly spliced transcript with the potential to encode the entire open reading frame. Spliced subgenomic transcripts representing ORF C mRNAs were not identified, suggesting that ORF C may be encoded from the full-length viral genomic transcript. We estimate that at least a 100-fold lower amount of the accessory/regulatory subgenomic transcripts exists in developing vs regressing tumors. These results demonstrate that WDSV undergoes an elaborate pattern of mRNA splicing similar to that of other complex retroviruses.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Retroviridae
/
Sarcoma Experimental
/
Neoplasias Cutáneas
/
ARN Mensajero
/
ARN Viral
/
Genoma Viral
/
Peces
/
Genes Virales
Límite:
Animals
Idioma:
En
Revista:
Virology
Año:
1997
Tipo del documento:
Article
País de afiliación:
Estados Unidos