An immuno-dot blot assay for detection of thermostable protease from Pseudomonas sp. AFT-36 of dairy origin.
Lett Appl Microbiol
; 25(4): 300-2, 1997 Oct.
Article
en En
| MEDLINE
| ID: mdl-9351281
ABSTRACT
A dot-ELISA technique for the detection of Pseudomonas protease was developed using IgG of anti-Pseudomonas AFT-36 protease as capture antibody. The detection limit of protease in buffer or milk was 1.01 ng ml-1. The procedure was performed at room temperature, took about 2.5 h and was economical. Protease AFT-36 is immunologically related to five out of seven Pseudomonas spp. The results suggest that the assay could be used to detect proteases in dairy products.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Endopeptidasas
/
Pseudomonas
/
Ensayo de Inmunoadsorción Enzimática
/
Productos Lácteos
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Límite:
Animals
Idioma:
En
Revista:
Lett Appl Microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
1997
Tipo del documento:
Article
País de afiliación:
India