An aldosterone regulated chicken intestine protein with high affinity to amiloride.
Comp Biochem Physiol A Physiol
; 118(2): 201-8, 1997 Oct.
Article
en En
| MEDLINE
| ID: mdl-9366044
ABSTRACT
The pattern of chicken intestine amiloride-binding proteins was determined using the photoreactive amiloride analogue 2'-methoxy-5'-nitrobenzamil (NMBA) and a polyclonal anti-amiloride antibody. At 10(-7)M, NMBA inhibits approximately 62% of the Na+ channel activity. At this concentration the amiloride analogue labels a number of membrane proteins, and in particular a 40-45 kDa polypeptide denoted ABP40. Incorporation of NMBA into ABP40 could be prevented by a 100-fold excess of benzamil, but not by a 1000-fold excess of 5-(N-ethyl-N-isopropyl)-amiloride. Labeling of ABP40 was intense in membranes derived from salt-deprived chickens and approximately 5-fold weaker in membranes from salt-repleted animals. Because of its small size, ABP40 is not likely to be an avian Na+ channel subunit, yet this amiloride-binding protein could be involved in the response to aldosterone.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Pollos
/
Diuréticos
/
Aldosterona
/
Amilorida
/
Mucosa Intestinal
/
Proteínas de la Membrana
Límite:
Animals
Idioma:
En
Revista:
Comp Biochem Physiol A Physiol
Asunto de la revista:
FISIOLOGIA
Año:
1997
Tipo del documento:
Article
País de afiliación:
Israel