Etomidate inhibits [3H]noradrenaline release from SH-SY5Y human neuroblastoma cells.
Neurosci Lett
; 236(2): 87-90, 1997 Oct 31.
Article
en En
| MEDLINE
| ID: mdl-9404818
ABSTRACT
We have examined the effects of the intravenous anaesthetic induction agent etomidate on K+ and carbachol evoked [3H]noradrenaline ([3H]NA) release and the associated increase in [Ca2+]i in SH-SY5Y human neuroblastoma cells in a attempt to study potential anaesthetic target site(s). Preincubation with etomidate produced a dose-dependent inhibition of both K+ and carbachol evoked [3H]NA release with estimated IC50 values of 88 and 69 microM, respectively. Only K+ stimulated increase in [Ca2+]i was inhibited by etomidate preincubation with an IC50 of 146 microM. Acute addition of etomidate after K+ challenge also inhibited the increase in [Ca2+]i with an IC50 of 99 microM. In addition etomidate displaced the binding of [3H]PN200-110 to L-type voltage sensitive Ca2+ channels with a Ki of 48 microM. As K+ but not carbachol evoked [3H]NA release is extracellular Ca2+ dependent and was inhibited by etomidate these data coupled with the PN200-110 displacement studies suggest that etomidate may interact with L-type voltage sensitive Ca2+ channels. The inhibition of carbachol evoked release without affecting the associated increase in [Ca2+]i suggests that etomidate may exert additional effects at either the muscarinic receptor or the secretory machinery in these cells.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Norepinefrina
/
Etomidato
/
Neuroblastoma
Límite:
Humans
Idioma:
En
Revista:
Neurosci Lett
Año:
1997
Tipo del documento:
Article
País de afiliación:
Reino Unido