Entropic exclusion by neurofilament sidearms: a mechanism for maintaining interfilament spacing.
Biochemistry
; 36(49): 15035-40, 1997 Dec 09.
Article
en En
| MEDLINE
| ID: mdl-9424114
ABSTRACT
A long-range repulsive force near isolated neurofilaments was detected by exclusion of large molecules and by direct force measurements with atomic force microscopy. Adsorption of isolated native neurofilaments to a solid substrate in a high-salt solution (170 mM NaCl), in the presence of coisolating contaminants, shows that the contaminants are excluded from a zone that extends from 50-100 nm from the core of the filament. Force-distance measurements by AFM show the presence of a weak repulsive force that extends >50 nm from the core of the filament; this repulsive force is absent in homopolymers of neurofilament L or trypsinized native filaments that lack the long sidearms present in native filaments. These results suggest that neurofilament sidearms form an entropic brush, thereby providing a mechanism for maintaining interfilament spacing.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neurofibrillas
Límite:
Animals
Idioma:
En
Revista:
Biochemistry
Año:
1997
Tipo del documento:
Article
País de afiliación:
Estados Unidos