Crystal structure of a bacteriophage T7 DNA replication complex at 2.2 A resolution.
Nature
; 391(6664): 251-8, 1998 Jan 15.
Article
en En
| MEDLINE
| ID: mdl-9440688
ABSTRACT
DNA polymerases change their specificity for nucleotide substrates with each catalytic cycle, while achieving error frequencies in the range of 10(-5) to 10(-6). Here we present a 2.2 A crystal structure of the replicative DNA polymerase from bacteriophage T7 complexed with a primer-template and a nucleoside triphosphate in the polymerase active site. The structure illustrates how nucleotides are selected in a template-directed manner, and provides a structural basis for a metal-assisted mechanism of phosphoryl transfer by a large group of related polymerases.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Bacteriófago T7
/
ADN Polimerasa Dirigida por ADN
Idioma:
En
Revista:
Nature
Año:
1998
Tipo del documento:
Article
País de afiliación:
Estados Unidos