Silver-ion high-performance liquid chromatographic separation and identification of conjugated linoleic acid isomers.
Lipids
; 33(2): 217-21, 1998 Feb.
Article
en En
| MEDLINE
| ID: mdl-9507244
ABSTRACT
This is the first report of the application of silver-ion impregnated high-performance liquid chromatography (Ag(+)-HPLC) to the separation of complex mixtures of conjugated linolenic acid (CLA) isomers present in commercial CLA sources and foods and in biological specimens. This method showed a clear separation of CLA isomers into three groups related to their trans,trans, cis,trans or trans,cis, and cic,cis configuration of the conjugated double-bond system. In addition, this method separated individual positional isomers of the conjugated diene system within each geometrical isomeric group. Following Ag(+)-HPLC isolation, gas chromatography (GC)-electron impact mass spectrometry, and GC-direct deposition-Fourier transformed infrared spectroscopy were used to confirm the identity of two major positional isomers in the cis/trans region, i.e., delta 8,10- and delta 11,13-octadecadienoic acids, which had not been chromatographically resolved previously. Furthermore, the potential of this method was demonstrated by showing different Ag(+)-HPLC profiles exhibiting patterns of isomeric distributions for biological specimens from animals fed a diet containing a commercial CLA preparation, as well as for a commercial cheese product.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Plata
/
Ácidos Linoleicos
/
Cromatografía Líquida de Alta Presión
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Lipids
Año:
1998
Tipo del documento:
Article
País de afiliación:
Estados Unidos