Chemiluminescent measurement of oxalate in serum by detection of hydrogen peroxide generated through oxalate oxidase.
J Biolumin Chemilumin
; 12(6): 295-8, 1997.
Article
en En
| MEDLINE
| ID: mdl-9509337
ABSTRACT
Today, chemiluminescence detection reactions have become popular in analytical biochemistry essentially due to their high sensitivity. A chemiluminescent synthetic system (luminol/porphyrin) was successfully used to measure serum oxalate by determination of hydrogen peroxide generated through oxalate oxidase (EC 1.2.3.4.). This new method is efficient and simple, highly sensitive and the results obtained in normal adult subjects are in good agreement with those of approved methods. This original application of such a chemiluminescent system allowed us to achieve a sensitive serum oxalate assay (detection limit of 0.2 mumol/L) characterized by a low serum volume (200 microL) required for analysis.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oxalatos
/
Oxidorreductasas
/
Peróxido de Hidrógeno
Tipo de estudio:
Diagnostic_studies
Límite:
Adult
/
Humans
Idioma:
En
Revista:
J Biolumin Chemilumin
Asunto de la revista:
BIOFISICA
/
BIOQUIMICA
Año:
1997
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
ENGLAND
/
ESCOCIA
/
GB
/
GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
/
UK
/
UNITED KINGDOM