Indirect glutamate neurotoxicity.
J Neurotrauma
; 15(2): 141-7, 1998 Feb.
Article
en En
| MEDLINE
| ID: mdl-9512089
ABSTRACT
Indirect glutamate toxicity can be demonstrated by exposing dissociated rat hippocampal cultures to the media of the same culture transiently exposed (1 min) to glutamate (0.5 mM). The toxicity was maximum when the media was collected 5 min after the glutamate exposure. While the primary glutamate toxicity was attenuated by ionotropic glutamate receptor antagonists, the transferred, indirect toxicity was unaffected by the same antagonists. The changes in nuclear morphology indicated chromatin condensation and nuclear fragmentation in both primary and transferred toxicity. The stain for DNA damage by TUNEL method also revealed cells staining positive in both primary and transferred glutamate toxicity. These observations demonstrate that glutamate-induced neurotoxicity can be propagated to the uninjured cells by an unknown toxin released into the extracellular space. This neurotoxin induced both apoptosis and necrosis in cultured rat hippocampal cells.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ácido Glutámico
/
Hipocampo
Límite:
Animals
Idioma:
En
Revista:
J Neurotrauma
Asunto de la revista:
NEUROLOGIA
/
TRAUMATOLOGIA
Año:
1998
Tipo del documento:
Article
País de afiliación:
Estados Unidos