Macrophage-colony stimulating factor (M-CSF) regulates the expression of fibronectin and its alpha5 integrin receptor in human trophoblasts.
Endocrinology
; 139(4): 2190-3, 1998 Apr.
Article
en En
| MEDLINE
| ID: mdl-9529009
ABSTRACT
Macrophage-colony stimulating factor (M-CSF, CSF-1) is secreted by human trophoblasts as well as endometrial cells, while its receptor c-fms is abundantly expressed by the extravillous trophoblasts anchoring the placenta to the uterus, suggesting a role for M-CSF at the maternal-fetal interface. We investigated the effect of M-CSF on the expression of fibronectin and its receptor, the alpha5 integrin, in human trophoblasts. Exposure of trophoblasts to M-CSF produced a two to three-fold increase in fibronectin and alpha5 mRNA abundance at both 24 and 72 hours of culture. A dose-dependent increase in cellular fibronectin secretion into the culture medium was detected at both time points. Immunocytochemistry showed co-localization of cellular fibronectin and alpha5 in the cells, suggesting that attachment of trophoblasts to fibronectin is mediated in part by the alpha5beta1 integrin. We conclude that M-CSF increases fibronectin expression and secretion by the human trophoblasts, and up-regulates its specific receptor, the alpha5 integrin. We hypothesize that M-CSF may partake in the autocrine/paracrine mechanisms regulating trophoblast invasion during implantation.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Trofoblastos
/
Antígenos CD
/
Regulación de la Expresión Génica
/
Factor Estimulante de Colonias de Macrófagos
/
Fibronectinas
Límite:
Female
/
Humans
/
Pregnancy
Idioma:
En
Revista:
Endocrinology
Año:
1998
Tipo del documento:
Article
País de afiliación:
Estados Unidos