The nitric oxide precursor L-arginine reduces expression of hyaluronan synthase in experimental vein bypass grafts.
J Surg Res
; 74(1): 39-42, 1998 Jan.
Article
en En
| MEDLINE
| ID: mdl-9536971
ABSTRACT
BACKGROUND:
The success of vascular bypass procedures is limited by the development of intimal hyperplasia (IH). The nitric oxide (NO) precursor, L-arginine (L-ARG) significantly reduces IH in both arteries and experimental vein grafts; however, the precise mechanism has yet to be elucidated. Hyaluronan synthase-1 (HAS-1) is one of the two enzymes believed to be responsible for making hyaluronan, a key component extracellular matrix composition.PURPOSE:
To determine how L-ARG supplementation affects the gene expression of HAS-1 in experimental vein grafts.METHODS:
Thirty-four male New Zealand white rabbits were divided into three groups control (no operation, regular chow and water, n = 4); L-ARG supplemented (n = 15); and no L-ARG (n = 15). The latter two groups underwent a right interposition carotid bypass using jugular vein. Vein grafts were harvested at 7, 14, and 21 days after surgery. Ribonuclease protection assays were performed using 32P-labeled riboprobes for HAS-1 and 18S rRNA as an internal control and expressed as a ratio (HAS-1/rRNA).RESULTS:
There was a significant rise in HAS-1 expression in the vein grafts 7 (1.57 +/- 0.5), 14 (0.7 +/- 0.2), and 21 days (2.82 +/- 0.7) after grafting compared to control (0.14 +/- 0.08) (P < 0.05). L-ARG-supplemented animals had a significant decrease in HAS-1 expression at 21 days (0.65 +/- 0.1) compared to nonsupplemented vein grafts (2.82 +/- 0.7) (P < 0.02).CONCLUSIONS:
These results demonstrate for the first time a significant rise in HAS expression in the early experimental vein grafts. Furthermore, L-ARG supplementation significantly diminishes the expression of HAS at 21 days. These results may represent a potential mechanism by which augmentation of the L-ARG/NO pathway inhibits IH in experimental vein grafts and may ultimately provide for improved therapeutic interventions in alleviating IH.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Arginina
/
Transferasas
/
Glicosiltransferasas
/
Glucuronosiltransferasa
/
Proteínas de Xenopus
/
Isoenzimas
/
Venas Yugulares
/
Proteínas de la Membrana
/
Óxido Nítrico
Tipo de estudio:
Etiology_studies
Límite:
Animals
Idioma:
En
Revista:
J Surg Res
Año:
1998
Tipo del documento:
Article
País de afiliación:
Estados Unidos