Detection of Yersinia enterocolitica in food by PCR amplification.
Lett Appl Microbiol
; 26(2): 140-4, 1998 Feb.
Article
en En
| MEDLINE
| ID: mdl-9569698
ABSTRACT
A polymerase chain reaction (PCR) assay was developed for detection of pathogenic, virulent strains of Yersinia enterocolitica. By using both virulence loci virF and ail as markers for pathogenicity, detection of species with a virulence factor present was possible. DNA preparation in the presence of hexadecyl trimethy ammonium bromide (CTAB) was followed by two 44 cycle amplification reactions, one for each of the markers. As few as 10(2) Y. enterocolitica cells were detected in ground pork in the presence of 10(5)-10(6) bacteria of other species. The described PCR assay provides a sensitive robust assay for the detection of virulent Y. enterocolitica in food.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Yersinia enterocolitica
/
Reacción en Cadena de la Polimerasa
/
Microbiología de Alimentos
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Lett Appl Microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
1998
Tipo del documento:
Article
País de afiliación:
Suecia