A miniaturized monolayer trough with variable surface area in the square-millimeter range.
Biochim Biophys Acta
; 1371(2): 345-50, 1998 May 28.
Article
en En
| MEDLINE
| ID: mdl-9630718
ABSTRACT
A new simple concept for a miniaturized monolayer trough is described. The overall monolayer area in the expanded state is approximately 150 mm2 and can be reduced by a factor of 2. The surface area is a function of the shape of the meniscus formed by the subphase and is controlled by the amount of water in the monolayer trough. The controlled compression of monolayers to a desired area per molecule with simultaneous observation of the lateral distribution of fluorescently labeled molecules is shown. A biological reaction between a specific antibody and lipid anchored peptide demonstrates the feasibility of monolayer experiments, which require only very small quantities of substance (in the pmol range). This trough might also be a valuable tool for the 2D crystallization of proteins at lipid layers via specific binding sites such as metal chelators.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Lípidos de la Membrana
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
1998
Tipo del documento:
Article
País de afiliación:
Suiza
Pais de publicación:
HOLANDA
/
HOLLAND
/
NETHERLANDS
/
NL
/
PAISES BAJOS
/
THE NETHERLANDS