Interactions of DNA with fluorescent dyes: by scanning tunneling microscopy.
Int J Biol Macromol
; 23(1): 7-10, 1998 Jul.
Article
en En
| MEDLINE
| ID: mdl-9644591
Genomic DNA was obtained from peripheral blood samples of healthy volunteers and interacted with two fluorescent dyes (i.e. Hoechst 33,258 and ethidium bromide) in aqueous media. These media containing DNA-dye complexes deposited on the gold coated mica surfaces. Then, STM images were obtained in which the STM was operated in air at atmospheric pressure with a tip-to-substrate bias voltage of 250-1000 mV (sample positive) and the tunneling currents in the range of 10-20 pA by using etched tips of Pt/Ir, in constant current mode. Both dyes from molecular clusters on DNA. While, the Hoechst molecules were observed on the DNA chains at regular distances, the ethidium bromide molecular clusters did not.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ADN
/
Microscopía de Túnel de Rastreo
/
Colorantes Fluorescentes
Límite:
Humans
Idioma:
En
Revista:
Int J Biol Macromol
Año:
1998
Tipo del documento:
Article
País de afiliación:
Turquía
Pais de publicación:
Países Bajos