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Interactions of DNA with fluorescent dyes: by scanning tunneling microscopy.
Zareie, M H; Sahin, F I; Ergün, M A; Koçum, C; Menevse, S; Menevse, A; Piskin, E.
Afiliación
  • Zareie MH; Chemical Engineering Department, Hacettepe University, Beytepe, Ankara, Turkey.
Int J Biol Macromol ; 23(1): 7-10, 1998 Jul.
Article en En | MEDLINE | ID: mdl-9644591
Genomic DNA was obtained from peripheral blood samples of healthy volunteers and interacted with two fluorescent dyes (i.e. Hoechst 33,258 and ethidium bromide) in aqueous media. These media containing DNA-dye complexes deposited on the gold coated mica surfaces. Then, STM images were obtained in which the STM was operated in air at atmospheric pressure with a tip-to-substrate bias voltage of 250-1000 mV (sample positive) and the tunneling currents in the range of 10-20 pA by using etched tips of Pt/Ir, in constant current mode. Both dyes from molecular clusters on DNA. While, the Hoechst molecules were observed on the DNA chains at regular distances, the ethidium bromide molecular clusters did not.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / Microscopía de Túnel de Rastreo / Colorantes Fluorescentes Límite: Humans Idioma: En Revista: Int J Biol Macromol Año: 1998 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Países Bajos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / Microscopía de Túnel de Rastreo / Colorantes Fluorescentes Límite: Humans Idioma: En Revista: Int J Biol Macromol Año: 1998 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Países Bajos