Determination of lycopene, alpha-carotene and beta-carotene in serum by liquid chromatography-atmospheric pressure chemical ionization mass spectrometry with selected-ion monitoring.
J Chromatogr B Biomed Sci Appl
; 708(1-2): 67-73, 1998 Apr 24.
Article
en En
| MEDLINE
| ID: mdl-9653948
ABSTRACT
A selected-ion monitoring (SIM) determination of serum lycopene, alpha-carotene and beta-carotene by an atmospheric pressure chemical ionization mass spectrometry (APCI-MS) was developed. A large amount of serum cholesterols disturbed the SIM determination of carotenoids by contaminating the segment of interface with the LC-MS. Therefore, separation of carotenoids from the cholesterols was performed using a mixed solution of methanol and acetonitrile (7030) as the mobile phase on a C18 column of mightsil ODS-5 (75 mm x 4.6 mm I.D.). The SIM determination was carried out by introducing only the peak portions of carotenoids and I.S. (squalene) by means of an auto switching valve. In the positive mode of APCI-MS, lycopene, alpha-carotene and beta-carotene were monitored at m/z 537 and I.S. was monitored at m/z 411. This method was linear for all analytes in the range of 15-150 ng for lycopene, 7-70 ng for alpha-carotene and 25-50 ng for beta-carotene. The detection limit of LC-APCI-MS-SIM for carotenoids was about 3 ng per 1 ml of serum (S/N = 3). The repeatabilities, expressed as C.V.s, were 10%, 8.4% and 5.3% for lycopene, alpha-carotene and beta-carotene, respectively. The intermediate precisions, expressed as C.V.s, were 11.2%, 8.8% and 6.5% for lycopene, alpha-carotene and beta-carotene, respectively.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Espectrometría de Masas
/
Carotenoides
/
Beta Caroteno
Límite:
Humans
Idioma:
En
Revista:
J Chromatogr B Biomed Sci Appl
Asunto de la revista:
QUIMICA CLINICA
Año:
1998
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
HOLANDA
/
HOLLAND
/
NETHERLANDS
/
NL
/
PAISES BAJOS
/
THE NETHERLANDS