Two-dimensional electrophoresis of proteins in an immobilized pH 4-12 gradient.
Electrophoresis
; 19(8-9): 1516-9, 1998 Jun.
Article
en En
| MEDLINE
| ID: mdl-9694305
ABSTRACT
For checking theoretical two-dimensional (2-D) maps derived from sequenced genomes, indicating that nonnegligible amounts of proteins up to pH 12 are to be expected, a wide-range immobilized pH 4-12 gradient was generated. Depending on the extraction method of sample preparation, proteins with pls up to pH 12 are detected in a single gel. Highly reproducible protein patterns focused to the steady state with round-shaped spots up to pH 12 are obtained with the standard protocol originally described in 1988 (Görg et al., Electrophoresis 1988, 9, 531-546).
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Electroforesis en Gel Bidimensional
/
Proteínas
Idioma:
En
Revista:
Electrophoresis
Año:
1998
Tipo del documento:
Article
País de afiliación:
Alemania