Sequence-independent amplification and cloning of large dsRNA virus genome segments by poly(dA)-oligonucleotide ligation.
J Virol Methods
; 72(2): 243-7, 1998 Jun.
Article
en En
| MEDLINE
| ID: mdl-9694332
ABSTRACT
A strategy was developed for sequence-independent synthesis and amplification of full-length cDNA of 3-4 kb genes of dsRNA viruses. The method of single primer amplification (Lambden et al., 1992) was adapted by the inclusion of a 3' poly(A) tail to an oligonucleotide ligated to dsRNA genome segments as a template for oligo(dT)-primed cDNA synthesis. Full-length copies of the largest genome segments, 1 (4 kb) and 2 (3 kb), of African horse sickness virus (AHSV) have been cloned, terminally sequenced and expressed in vitro.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Virus ARN
/
Amplificación de Genes
/
ADN Complementario
Límite:
Animals
Idioma:
En
Revista:
J Virol Methods
Año:
1998
Tipo del documento:
Article
País de afiliación:
Sudáfrica