Correlation of chi orientation with transcription indicates a fundamental relationship between recombination and transcription.
Gene
; 216(2): 285-92, 1998 Aug 31.
Article
en En
| MEDLINE
| ID: mdl-9729432
ABSTRACT
Cross-over hot-spot instigator (Chi) sequences (5'-GCTGGTGG-3') are abundant, strand-specific, sequences, which locally increase recombination in Escherichia coli. Located within G-rich 'recombination islands', Chi orientations correlate with the orientations both of DNA replication and of transcription. Consistent with evidence from eukaryotic systems for a fundamental relationship between recombination and transcription, we find for E. coli Chi sequences, and for Haemophilus influenzae Chi-like sequences, that orientations correlate better with transcription than with replication. Complying with Szybalski's transcription direction rule, open reading frames in these prokaryotes have purine-rich mRNA-synonymous DNA strands. Hence, the G-richness of 'recombination islands' may reflect their correspondence with 'transcriptional islands' (genes). Comparison of a natural with the corresponding shuffled sequence, indicates a base order-dependent island unit of approx. 1kb. 1998 Elsevier Science B.V.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Recombinación Genética
/
Transcripción Genética
/
Haemophilus influenzae
/
Intercambio Genético
/
Escherichia coli
Idioma:
En
Revista:
Gene
Año:
1998
Tipo del documento:
Article
País de afiliación:
Canadá