Antisense oligodeoxyribonucleotide against the MLL-LTG19 chimeric transcript inhibits cell growth and induces apoptosis in cells of an infantile leukemia cell line carrying the t(11;19) chromosomal translocation.
Cancer Res
; 58(17): 3773-6, 1998 Sep 01.
Article
en En
| MEDLINE
| ID: mdl-9731482
ABSTRACT
To clarify the role of the multiple lineage leukemia gene-leukemia translocation gene of chromosome 19 (MLL-LTG19) protein in leukemogenesis, we synthesized antisense oligodeoxyribonucleotide (ODN) against the fused region of the MLL-LTG19 chimeric transcript and treated KOCL33 cells carrying the t(11;19) translocation with antisense ODN. The antisense ODN inhibited cell growth and induced apoptosis in KOCL33 cells but not in Daudi cells, which have no t(11;19). The levels of MLL-LTG19 mRNA and MLL-LTG19 protein in KOCL33 cells treated with antisense ODN were shown to decrease with time by reverse transcription-PCR and Western blot analysis. These results suggest that the MLL-LTG19 fusion protein contributes to cell proliferation and malignant transformation in infantile acute leukemia cells carrying the t(11;19) translocation.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Factores de Transcripción
/
Translocación Genética
/
Cromosomas Humanos Par 11
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Cromosomas Humanos Par 19
/
Proteínas Recombinantes de Fusión
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Proto-Oncogenes
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Leucemia de Células B
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Oligonucleótidos Antisentido
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Apoptosis
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Proteínas de Unión al ADN
Límite:
Humans
Idioma:
En
Revista:
Cancer Res
Año:
1998
Tipo del documento:
Article
País de afiliación:
Japón