GFR alpha-4 and the tyrosine kinase Ret form a functional receptor complex for persephin.
Curr Biol
; 8(18): 1019-22, 1998 Sep 10.
Article
en En
| MEDLINE
| ID: mdl-9740802
ABSTRACT
Glial-cell-line-derived neurotrophic factor (GDNF), neurturin and persephin are structurally related, secreted proteins that are widely expressed in the nervous system and other tissues and promote the survival of a variety of neurons during development. GDNF and neurturin signal through multicomponent receptors that consist of the Ret receptor tyrosine kinase and one of two structurally related glycosyl-phosphatidylinositol (GPI)-linked ligand-binding subunits GFR alpha-1 is the preferred ligand-binding subunit for GDNF, and GFR alpha-2 is the preferred ligand-binding subunit for neurturin. Two additional members of the GFR alpha family of GPI-linked proteins have recently been cloned GFR alpha-3 and GFR alpha-4. We have shown that persephin binds efficiently only to GFR alpha-4, and labelled persephin is effectively displaced from cells expressing GFR alpha-4 by persephin but not by GDNF or neurturin. Using microinjection to introduce expression plasmids into cultured neurons, we have also shown that coexpression of Ret with GFR alpha-4, confers a marked survival response to persephin but not to GDNF or neurturin. These results demonstrate that GFR alpha-4 is the ligand-binding subunit for persephin and that persephin, like GDNF and neurturin, also requires Ret for signalling.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Glicoproteínas de Membrana
/
Proteínas Proto-Oncogénicas
/
Receptores de Factor de Crecimiento Nervioso
/
Proteínas Tirosina Quinasas Receptoras
/
Receptores de Superficie Celular
/
Receptor Cross-Talk
/
Proteínas de Drosophila
/
Factores de Crecimiento Nervioso
/
Proteínas del Tejido Nervioso
Límite:
Humans
Idioma:
En
Revista:
Curr Biol
Asunto de la revista:
BIOLOGIA
Año:
1998
Tipo del documento:
Article
País de afiliación:
Reino Unido