Cell penetrating PNA constructs regulate galanin receptor levels and modify pain transmission in vivo.
Nat Biotechnol
; 16(9): 857-61, 1998 Sep.
Article
en En
| MEDLINE
| ID: mdl-9743120
ABSTRACT
Peptide nucleic acids (PNAs) form stable and tight complexes with complementary DNA and/or RNA and would be promising antisense reagents if their cellular delivery could be improved. We show that a 21-mer PNA, complementary to the human galanin receptor type 1 mRNA, coupled to the cellular transporter peptides, transportan or pAntennapedia(43-58), is efficiently taken up into Bowes cells where they block the expression of galanin receptors. In rat, the intrathecal administration of the peptide-PNA construct results in a decrease in galanin binding in the dorsal horn. The decrease in binding results in the inability of galanin to inhibit the C fibers stimulation-induced facilitation of the rat flexor reflex, demonstrating that peptide-PNA constructs act in vivo to suppress expression of functional galanin receptors.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Dolor
/
Factores de Transcripción
/
Proteínas Nucleares
/
Transducción de Señal
/
Receptores de Neuropéptido
/
Ácidos Nucleicos de Péptidos
Límite:
Animals
/
Female
/
Humans
Idioma:
En
Revista:
Nat Biotechnol
Asunto de la revista:
BIOTECNOLOGIA
Año:
1998
Tipo del documento:
Article
País de afiliación:
Suecia