Your browser doesn't support javascript.
loading
[Effects of HGF on the production of matrix metalloproteinases by colonic cancer cells DHD/K12]. / Effets de l'HGF sur la production de métalloprotéinases matricielles par les cellules cancéreuses coliques DHD/K12.
Aparicio, T; Kermorgant, S; Lewin, M J; Lehy, T.
Afiliación
  • Aparicio T; INSERM U10 et IFR cellules épithéliales, Hôpital Bichat-Claude-Bernard, Paris.
C R Seances Soc Biol Fil ; 192(2): 311-5, 1998.
Article en Fr | MEDLINE | ID: mdl-9759374
Matrix metalloproteinases (MMPs) and growth factors such as hepatocyte growth factor (HGF) are implicated in tumoral progression of several digestive cancers. The rat DHD/K12 colonic cancer cell line is very invasive in vivo. We showed by RT-PCR and western immunoblotting the presence of HGF receptor, c-Met, in DHD/K12 cells. Then, we detected by zymography and western blots the secretion of MMP-2 and MMP-9 in the conditioned medium of these cells. After 24 or 48 h of culture in medium supplemented with HGF, transforming growth factor-alpha (TGF-alpha) or sodium butyrate, MMP production by DHD/K12 cells was stimulated by HGF and TGF-alpha and inhibited by sodium butyrate. Knowing the capacity of MMPs to degrade the extracellular matrix and thus to favour tumoral invasion, results suggest that HGF is implicated in the aggressive behaviour of DHD/K12 cells since it increased MMPs secretion by these cells.
Asunto(s)
Buscar en Google
Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Metaloendopeptidasas / Factor de Crecimiento de Hepatocito Límite: Animals Idioma: Fr Revista: C R Seances Soc Biol Fil Año: 1998 Tipo del documento: Article Pais de publicación: Francia
Buscar en Google
Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Metaloendopeptidasas / Factor de Crecimiento de Hepatocito Límite: Animals Idioma: Fr Revista: C R Seances Soc Biol Fil Año: 1998 Tipo del documento: Article Pais de publicación: Francia