ATP-dependent copper transport by the Menkes protein in membrane vesicles isolated from cultured Chinese hamster ovary cells.
FEBS Lett
; 435(2-3): 178-82, 1998 Sep 18.
Article
en En
| MEDLINE
| ID: mdl-9762903
ABSTRACT
The Menkes (MNK) protein is a vital component of copper homeostasis in mammalian cells. In this paper we provide the first biochemical evidence that the MNK protein functions as a copper-translocating P-type ATPase in mammalian cells. The enzyme activity in membrane vesicles prepared from Chinese hamster ovary cells overexpressing MNK was ATP-dependent, correlated with the amount of MNK and followed Michaelis-Menten kinetics with respect to copper. The copper transport was observed only under reducing conditions suggesting MNK transports Cu(I). This study opens the way to detailed structure-function studies and assessment of functional MNK derived from patients with Menkes disease.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Recombinantes de Fusión
/
Proteínas Portadoras
/
Membrana Celular
/
Adenosina Trifosfatasas
/
Cobre
/
Gránulos Citoplasmáticos
/
Proteínas de Transporte de Catión
Límite:
Animals
Idioma:
En
Revista:
FEBS Lett
Año:
1998
Tipo del documento:
Article