Antigenic characterization of canine parvovirus strains isolated in Italy.
J Virol Methods
; 73(2): 197-200, 1998 Aug.
Article
en En
| MEDLINE
| ID: mdl-9766890
ABSTRACT
28 isolates of canine parvovirus type-2 (CPV-2) were obtained from dogs with hemorrhagic gastroenteritis in Italy. The antigenic structure of CPV-2 isolates was characterized, using four discriminating monoclonal antibodies. In addition, four vaccinal strains were examined. Similar to reports from Australia and the United Kingdom, a much higher prevalence of CPV-2a (25/28 isolates) was observed than the other variant type, CPV-2b (3/28 isolates). DNA fragments (2.2 kbp) of representative strains of CPV-2, CPV-2a and CPV-2b were amplified by the polymerase chain reaction (PCR) and the products were digested by the restriction enzymes (RE) RsaI, HpaII, HindIII and PvuII. The RvaI enzyme allows the differentiation of CPV-2 from CPV-2a and CPV-2b.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Parvovirus Canino
/
Infecciones por Parvoviridae
/
Antígenos Virales
Tipo de estudio:
Risk_factors_studies
Límite:
Animals
País/Región como asunto:
Europa
Idioma:
En
Revista:
J Virol Methods
Año:
1998
Tipo del documento:
Article
País de afiliación:
Italia