Static time-of-flight secondary ion mass spectrometry imaging of freeze-fractured, frozen-hydrated biological membranes.
Rapid Commun Mass Spectrom
; 12(18): 1232-5, 1998.
Article
en En
| MEDLINE
| ID: mdl-9772765
ABSTRACT
The study of cell membrane lipid and steroid composition and distribution is important for the understanding of membrane dynamics and function. Here we present efforts to chemically image phospholipid distributions on a submicron scale on freeze-fractured and frozen-hydrated liposomes and red blood cells using time-of-flight secondary ion mass spectrometry. Sample preparation by freeze fracturing of membranes is described. Fragments representative of phospholipid headgroups are found to be localized on both liposomes and red blood cells. In addition, the cholesterol molecular ion [M + H] is localized on liposome surfaces.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Membranas
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Rapid Commun Mass Spectrom
Año:
1998
Tipo del documento:
Article
País de afiliación:
Estados Unidos