Developmental potential of bovine embryos reconstructed from enucleated matured oocytes fused with cultured somatic cells.
C R Acad Sci III
; 321(9): 735-45, 1998 Sep.
Article
en En
| MEDLINE
| ID: mdl-9809205
ABSTRACT
Muscle and skin biopsies taken from bovine fetuses and young calves have been used as a source of donor nuclei for cloning experiments. After culture, cells were individually fused to enucleated matured oocytes and the resulting blastocysts obtained after 7 d of culture (3-8% depending on the cell type) were transferred to foster recipient heifers. Two calves, a female and a male, both originating from muscle cells were born, and four additional pregnancies have surpassed mild-term gestation. The pregnancies include one fetus established from a transgenic nucleus from a fetal skin cell, and another one resulting from a skin biopsy performed on a female calf. Our data demonstrate that nuclei from cultured bovine somatic cells obtained from differentiated tissues can be made multipotent.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oocitos
/
Fusión Celular
/
Desarrollo Embrionario y Fetal
Límite:
Animals
/
Pregnancy
Idioma:
En
Revista:
C R Acad Sci III
Asunto de la revista:
BIOLOGIA
Año:
1998
Tipo del documento:
Article
País de afiliación:
Francia