Purification, characterization and immunochemical properties of a novel 60-kDa protein of Vibrio anguillarum strains.
FEMS Microbiol Lett
; 168(1): 111-7, 1998 Nov 01.
Article
en En
| MEDLINE
| ID: mdl-9812371
ABSTRACT
Vibrio anguillarum strains expressed increased amounts of a novel 60-kDa protein when cells were grown at physiologically elevated temperatures. The relative amounts of the 60-kDa protein were unaltered by changes in osmolarity or ionic concentration of the growth medium in cells grown at optimal growth temperatures. The N-terminal amino acid sequence analysis of the V. anguillarum 60-kDa protein showed extensive (94-89%) sequence identity with the 60-kDa heat shock protein of Yersinia enterocolitica and with Serratia rubidaea GroEL protein. Monoclonal antibodies against the Y. enterocolitica chaperonin reacted with the 60-kDa protein from V. anguillarum strains, and with a temperature-induced protein of similar molecular mass in other Gram-negative pathogens of fish.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Bacterianas
/
Vibrio
/
Vibriosis
/
Enfermedades de los Peces
/
Proteínas de Choque Térmico
Límite:
Animals
Idioma:
En
Revista:
FEMS Microbiol Lett
Año:
1998
Tipo del documento:
Article
País de afiliación:
Canadá