Quantitation of active ingredients and excipients in nasal sprays by high-performance liquid chromatography, capillary electrophoresis and UV spectroscopy.
J Chromatogr A
; 823(1-2): 423-31, 1998 Oct 09.
Article
en En
| MEDLINE
| ID: mdl-9818419
ABSTRACT
A study on the use of different analytical methodologies to determine active ingredients and excipients found in commercial nasal sprays is presented. Two of the developed methodologies consisted of separation techniques, i.e. high-performance liquid chromatography and capillary electrophoresis, and the third one involved a UV-spectroscopic multicomponent procedure. The samples studied are characterized by a high viscosity and the existence of a large number of particles in suspension; therefore, special emphasis is paid on the sample preparation required by each methodology. Advantages and drawbacks of each analytical technique are also discussed in terms of speed of analysis, sensitivity and reproducibility. From this work it is observed that although the UV method needs the most laborious sample preparation, the total time required per analysis is the shortest one. The best reproducibility in terms of analysis time and quantitation of the analyzed compounds is obtained using HPLC. CE allows the determination of more components in the same sample.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Espectrofotometría Ultravioleta
/
Administración Intranasal
/
Cromatografía Líquida de Alta Presión
/
Electroforesis Capilar
/
Antiasmáticos
/
Antialérgicos
/
Excipientes
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
J Chromatogr A
Año:
1998
Tipo del documento:
Article
País de afiliación:
España