Amperometric biosensors based on solid binding matrices applied in food quality monitoring.
Biosens Bioelectron
; 13(7-8): 911-23, 1998 Oct 01.
Article
en En
| MEDLINE
| ID: mdl-9828388
ABSTRACT
Solid binding matrix (SBM) based composite transducers have been used for development of series of multibiosensor systems applicable in various fields. Here we present two hybrid three-channel multibiosensors for simultaneous amperometric operation in food quality control, i.e. glucose/fructose/ethanol multibiosensor, based on glucose oxidase/fructose dehydrogenase/alcohol dehydrogenase surface-modified enzyme electrodes and L-lactate/L-malate/sulfite multibiosensor, based on L-lactate dehydrogenase/L-malate dehydrogenase/sulfite oxidase surface-modified enzyme electrodes. Different parameters have been studied in order to optimize the response of the multibiosensor systems. The multibiosensor showed a good sensitivity, linear range and storage stability. The multibiosensors were used for the determination of glucose, fructose, ethanol, L-lactate, L-malate and sulfite in samples of wine, resulting in a good agreement with data certified by the supplier. Comparison of various designs, surface-modified, bulk-modified and thick-cover, of SBM based biosensors is studied on the example of fructose biosensor.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Técnicas Biosensibles
/
Análisis de los Alimentos
Idioma:
En
Revista:
Biosens Bioelectron
Asunto de la revista:
BIOTECNOLOGIA
Año:
1998
Tipo del documento:
Article
País de afiliación:
Italia