The P histo-blood group-related glycosphingolipid sialosyl galactosyl globoside as a preferred binding receptor for uropathogenic Escherichia coli: isolation and structural characterization from human kidney.
Biochemistry
; 37(50): 17420-8, 1998 Dec 15.
Article
en En
| MEDLINE
| ID: mdl-9860857
ABSTRACT
The P histo-blood group-related glycosphingolipid, sialosyl galactosyl globoside (SGG), has recently been implicated as a preferred binding receptor for uropathogenic Escherichia coli [Stapleton, A. E., Stroud, M. R., Hakomori, S., and Stamm, W. E. (1998) Infect. Immun. 66, 3856-3861]. We report here the purification and complete structural characterization of SGG from normal human kidney. Using metabolically [35S]-labeled E. coli as a probe, a monosialylated glycosphingolipid was isolated to homogeneity. The glycosphingolipid was purified by a combination of high-performance liquid chromatography and preparative high-performance thin-layer chromatography and its structure unambiguously elucidated by 1H NMR, electrospray ionization mass spectrometry, and methylation analysis. Its primary structure was shown to be identical to a previously characterized, developmentally regulated, globo-series glycolipid thought to be unique to human teratocarcinoma. The significance of this structure as a unique receptor in human kidney for uropathogenic E. coli and its role in the pathogenesis of urinary tract infections are discussed.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Sistema del Grupo Sanguíneo P
/
Infecciones Urinarias
/
Receptores de Superficie Celular
/
Escherichia coli
/
Infecciones por Escherichia coli
/
Gangliósidos
/
Riñón
Tipo de estudio:
Etiology_studies
Límite:
Humans
Idioma:
En
Revista:
Biochemistry
Año:
1998
Tipo del documento:
Article
País de afiliación:
Estados Unidos